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Session I
Nitrogen Monoxide (NO): Metabolism and Pathophysiology

Nitric oxide synthase isoforms possess structural and regulatory features which determine their unique functions
B.S.S. Masters, P. Martasek, L.J. Roman, R.T. Miller, C.S. Raman*, T.J. Poulos*,H. Li*, and J.S. Nishimura
University of Texas Health Science Center at San Antonio, TX and *University of California, Irvine, CA

Tetrahydrobiopterin availability limits high-output NO synthesis in vascular cells: therapeutic implications
Steven Gross
Cornell University Medical College, New York, NY 10021, USA

ATP- and GTP-dependent activation of iron regulatory protein-1 by oxidative stress
Kostas Pantopoulos and Matthias W. Hentze
European Molecular Biology Laboratory, Meyerhofstrasse 1 D-69117 Heidelberg, Germany

Regulation of mitochondrial oxygen uptake by O2 and NO
Alberto Boveris, Lidia E. Costa, and Juan J. Poderoso
Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

Reactive metabolites of oxygen and nitrogen, NF-kB, and chronic inflammation
Matthew B. Grisham
Department of Molecular and Cellular Physiology, LSU Medical Center Shreveport, LA


Session II
Skin, Oxidative Stress and Antioxidants

a-Tocopherol transfer protein: Regulatory mechanism for skin vitamin E?
M.G. Traber, J.J. Thiele, Y. Terasawa, R.V. Farese, Jr., W.L. Epstein, S. Weber, M. Podda, and L. Packer
Department of Molecular & Cell Biology, UC Berkeley, and UC San Francisco

Skin barrier a-tocopherol: A highly sensitive biomarker of environmental oxidative stress
Jens J. Thiele and Lester Packer
Department of Molecular & Cell Biology, UC Berkeley, USA

Modulation of UV-induced signal transduction in human keratinocytes by antioxidants
Claude Saliou, Laura McLaughlin, and Lester Packer
Department of Molecular and Cell Biology, University of California, Berkeley, California, USA

Signaling events by UVA radiation and singlet oxygen
Karlis Briviba, Lars-Oliver Klotz, Karin Scharffetter-Kochanek, Meinhard Wlaschek, Susanne Grether-Beck, Jean Krutmann and Helmut Sies
Institut für Physiologische Chemie I and Hautklinik, Heinrich-Heine-Universität Düsseldorf, and Hautklinik, Universität zu Köln, Germany

Possible roles for iron, heme oxygenase and ferritin in UVA-mediated oxidative stress and cell death
R.M. Tyrrell, C. Eden, V. Hejmadi, E. Kvam, C, Pourzand, S. Ryter, and A, Stacey
Department of Pharmacy and Pharmacology, University of Bath, BA2 7AY, Bath, United Kingdom

Antioxidants block the expression and functional activity of the skin-selective homing receptor cutaneous lymphocyte-associated antigen
Maurizio Podda, Sandra Diehl, Roland Kaufmann, Rainer Milbradt, Thomas M. Zollner
Department of Dermatology, University of Frankfurt Medical School, Germany

Cancer chemoprevention by polyphenolic antioxidants in green tea
Hasan Mukhtar, Sanjay Gupta, and Nihal Ahmad
Department of Dermatology, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, Ohio 44106, USA


Session III
Redox Regulation of Cell Signaling

Oxidant modulation of apoptotic cell death
Sten Orrenius
Institute of Environmental Medicine, Karolinska Institutet, Box 210, S-171 77 Stockholm, Sweden

Fas­mediated apoptosis: cellular events and lipoate regulation
Chandan K. Sen, Sashwati Roy, and Lester Packer
Department of Molecular and Cell Biology, University of California at Berkeley

Dissecting the signal transduction pathway for the activation of NF-kB by hydrogen peroxide
Kirsty McPherson and Christine Winterbourn
Free Radical Research Group, Department of Pathology, Christchurch School of Medicine, PO Box 4345, Christchurch, New Zealand

Redox regulation of NF-kB and novel therapeutic application
Takashi Okamoto
Department of Molecular Genetics, Nagoya City University, Nagoya, Japan

Oxidant-mediated signaling and the regulation of cell cycle progression and apoptosis in lung epithelial cells
Yvonne M.W. Janssen, Brooke T. Mossman, and Balaraman Kalyanaraman
Department of Pathology, University of Vermont, Burlington VT 05405 and the Biophysics Research Institute, Medical College of Wisconsin, Milwaukee WI 53226

A role of NF-kB/p53 interactions in cellular response to oxidative stress
Ken-ichi Yamamoto
Department of Molecular Pathology, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920, Japan

Hypochlorous acid increases p53 in cultured human skin cells: A mechanism by which bystander cells could escape the carcinogenicity associated with inflammation
Glenn F. Vile*, Lincoln A. Rothwell and Anthony J. Kettle
Free Radical Research Group, Department of Pathology Christchurch School of Medicine, P.O. Box 4345 Christchurch New Zealand

Redox events in the cytokine-induced expression of adhesion molecules in endothelial cells
Regina Brigelius-Flohé, Bärbel Friedrichs, Frank Tewes, and Gaby-Fleur Böl
German Institute of Human Nutrition, Potsdam-Rehbrücke, Germany

Redox control of Thioredoxin (TRX) on the cytotoxic/death signal
Junji Yodoi1, Shugo Ueda1,2, Masaya Ueno1,2, Tetsuro Sasada1, and Hiroshi Masutani1
1Department of Biological responses, Institute for Virus Research and 2Department of Gastroenterological Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan

Differential regulation of oxidative and hyperosmotic stress-induced Syk activation in B cells
Suofu Qin and Hirohei Yamamura
Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

Ascorbic acid and atherosclerosis
Takao Fujinami
Department of Internal Medicine, Nagoya City University Medical School, Nagoya, Japan

The distribution of glutathione peroxidases in testis suggests a dual role for selenium in the fertility of mammals
M. Maiorino, A. Roveri, L. Flohé* and F. Ursini
Department of Biological Chemistry, University of Padova, Padova, Italy and *Deptartment of Physiological Chemistry, Technical University of Braunschweig, Braunschweig, Germany

Oxidative damage precedes the formation of cytopathological lesions in Alzheimer disease
George Perry, Kiesuke Hirai, Lawrence M. Sayre, and Mark A. Smith
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106 USA

Oxidative damage to DNA and other biomolecules in neurodegenerative diseases
Barry Halliwell
Neurodegenerative Disease Research Centre, King¹s College, London, UK


Session IV
Natural Antioxidants: Molecular Mechanisms

Did the loss of endogenous ascorbate accelerate the evolution of Anthropoidea and Homo sapiens?
J. Challem
The Nutrition Reporter, PO Box 5505, Aloha, Oregon 97006

Iron overload and vitamin C: Pro- or anti-oxidant?
Balz Frei
Linus Pauling Institute, Oregon State University, 571 Weniger Hall, Corvallis, OR

Antioxidant interactions between vitamin E and coenzyme Q: Role of superoxide
Valerian E. Kagan
Departments of Environmental and Occupational Health and Pharmacology, University of Pittsburgh, Pittsburgh, PA 15238, USA

Actions of carotenoids
Norman I. Krinsky
Department of Biochemistry, School of Medicine and the Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA

Consideration for the efficacy of phenolic phytochemicals, flavonoids, and hydroxycinnamates as effective antioxidants
Catherine Rice-Evans
International Antioxidant Research Centre, UMDS-Guyís Hospital, London SE1 9RT, United Kingdom

Pycnogenol®: Effects on the redox antioxidant network and nitrogen monoxide (NO) metabolism
Lester Packer, Fabio Virgili, Hirotsugu Kobuchi, and Elaine Cossins
Department of Molecular and Cell Biology, University of California at Berkeley

EGb 761: Antioxidant properties and cell regulation
Sashwati Roy, Hirotsugu Kobuchi, Chandan K. Sen, Kishorchandra Gohil, Savita Khanna and Lester Packer
Membrane Bioenergetics Group, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200

Role of mitochondria in oxidative stress associated with aging and apoptosis. Studies in intact cells
José Viña
Department of Physiology, School of Medicine, University of Valencia, Valencia, Spain


Keynote Lecture

Cytochrome P450's as a source of oxygen radicals?
Ronald W. Estabrook
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75235-9038


Session VI
Oxidative Stress in Diabetes and Its Complications

Overview: Oxidative Stress, antioxidants, and diabetes
Lester Packer
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200

Evidence for increased carbonyl stress, but not increased oxidative stress in diabetes
John W. Baynes
University of South Carolina, Columbia, South Carolina

Antioxidant and provident profile in diabetes mellitus and its relevance to the onset of the syndrome
Jaffar Nourooz-Zadeh

Vitamin E deficiency in diabetic patients: How to measure, and relationship to resistance of LDL against oxidation
Wolfgang Leonhardt
Institute and Policlinic of Clinical Metabolic Research, Medical Faculty of the Technical University, Dresden, Germany

Endogenous antioxidants and lipoprotein oxidation in non insulin dependent diabetes mellitus (NIDDM)
Giorgio Bellomo, M. Bagnati, C. Cau, C. Perugini, P. Paffoni, R. Re, and E. Zoppis
Department of Medical Sciences, University of Torino, Novara, Italy

Glucose activates NF-kB and induces apoptosis in human endothelial cells by a NO-synthase dependent pathway
Peter Rösen*, Xueliang Du, Karin Färber
Diabetes Research Institute at the Heinrich-Heine-University, Düsseldorf, Germany

Vitamin E and selenium in the prevention of diabetes mellitus and rheumatoid arthritis
Paul Knekt1, Georg Alfthan1, Jukka Marniemi2, Aila Leino2, Antti Reunanen1, Markku Heliövaara1, and Kimmo Aho1
1National Public Health Institute, Helsinki, 2Social Insurance Institution, Turku, Finland

Specific inhibitory role of a-tocopherol in cell proliferation and its implications in the prevention of diabetis complications
A. Azzi, E. Aratri, D. Boscoboinik, S. Clément, A. Fazzio, N. K. Özer, R. Ricciarelli, and S. Spycher
Institut für Biochemie und Molekularbiologie, Universitaet Bern, Buehlstrasse 28- 3012 Bern, Switzerland

The role of antioxidants in the treatment of diabetic microangiopathy
Klaus Kusterer
Department of Medicine I, University of Frankfurt, Germany

Role of NFkB in the pathogenesis of late diabetic complications
P. Nawroth, A. Bierhaus, H. Tritschler, and R. Ziegler
Department of Medicine, University of Heidelber and Asta Medica AWD GmbH, Frankfurt

Diabetic neuropathy and nerve ischemia: Role of antioxidant defenses, lipid peroxidation, antioxidant treatment
P.A. Low, Y. Kishi, Y. Mitsui, M. Kishi, J. Tanabe, K.K. Nickander, J.D. Schmelzer, P.J. Zollman, and H.-J. Tritschler
Departments of Neurology, Mayo Clinic and Mayo Foundation, Rochester, MN, USA and Asta Medica, Frankfurt, Germany

Antioxidants in the treatment of neuropathic changes in diabetic rats: synergism with essential fatty acids
Norman E. Cameron and Mary A. Cotter
Department of Biomedical Sciences, Aberdeen University, Scotland, U.K.

How does vitamin E prevent the activation of diacylglycerol and protein kinase C in diabetic vascular tissues
G.L. King, D. Koya, I. Lee, and S. Burcell
Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215

In vivo activation of insulin signalling pathways by R (+) a-lipoic acid leads to translocation of glucose transporter to the plasma membrane of 3T3-L1 adipocytes
Karen Yaworsky, Romel Somwar, Toolsie Ramlal, Gary Sweeney, Phil Bilan, Hans Tritschler, and Amira Klip
The Hospital for Sick Children, Toronto, Ont., Canada M5G 1X8

a-Lipoic acid does not enhance insulin action on protein turnover in insulin-resistant rat skeletal muscle
Erik J. Henriksen1 & Randi B. Weinstein2
1Muscle Metabolism Laboratory, Department of Physiology, and 2Department of Biochemistry, University of Arizona College of Medicine, Tucson, AZ 85721-0093

A lipoic acid-g linolenic acid conjugate is effective against multiple indices of experimental diabetic neuropathy
Luke Hounsom, Roger Corder and David Tomlinson
Pharmacology, Queen Mary and Westfield College, Mile End, London E1 4NS, United Kingdom

Effects of dl-a-lipoic acid (LA) on diabetic nerve function, bioenergetics, and antioxidative defense
Douglas A. Greene, Xianghui Cao, Carol Van Huysen, and Irina G. Obrosova
Department of Internal Medicine, University of Michigan, Ann Arbor, MI

a-Lipoic acid in the treatment of diabetic neuropathy
Dan Ziegler
Diabetes Research Institute, Heinrich Heine University, Düsseldorf, Germany

Oxidative stress biomarkers in carcinogenesis and diabetes
Shinya Toyokuni
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan

Insulin sensitivity and antioxidants: Is there any interaction?
S. Jacob, K. Rett, and H.-U. Häring
Department of Endocrinology and Metabolism, University of Tübingen, Germany


Posters I

Mechanisms involved in Pb2+-mediated enhancement of lipid oxidation
Viviana N. Adonaylo and Patricia I. Oteiza
Departamento de Química Biológica, IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina

Determination of the chain-breaking rate constant of antioxidants in complex biological systems
Fernando Antunes1,2, Lawrence Ross Coates Barclay3 and Ruy E. Pinto1
1Grupo de Bioquímica e Biologia Teóricas, Instituto Bento da Rocha Cabral, P-1250 Lisboa, Portugal, 2Molecular Pharmacology ant Toxicology, University of Southern California, Los Angeles, California, USA, 3Mount Allison University, Department of Chemistry, Sackville, NB, Canada EOA 3CO

Effects of phospholipid composition on peroxidation of unsaturated fatty acyl chains of phospholipids in liposomes
K. Anzai, Y. Senzaki*, J. Ueda, Y. Miura, T. Ozawa, and Y. Matsushima*
National Institute of Radiological Sciences, Chiba, Japan and *Kyoritsu College of Pharmacy, Tokyo, Japan

DNA binding activities of transcription factors, NF-kB and AP-1 IN J774A-1 cell line in response to oxidative stress
M.C. Arkan*, S. Camandola§, G. Leonarduzzi, G. Poli§, and H. Basaga*
*Bogazici University, Department of Molecular Biology and Genetics, Istanbul, Turkey, and §Torino University, Department of General Pathology, Turin, Italy

Plant extracts as antioxidant prophylactic agents
Okezie I Aruoma
OICA International, P O Box MI 34, Micoud, Saint Lucia; and Pharmacology Group, King¹s College London, Manresa Road, London, SW3 6LX, UK

Regeneration of selenomethionine from methionine selenoxide by the low-molecular-weight reductants glutathione and ascorbate
Annika Assmann, Karlis Briviba, and Helmut Sies
Heinrich-Heine-Universität Düsseldorf, Institut für Physiologische Chemie I, Postfach 101007, D-40001 Düsseldorf, Germany

Structural-dependence of flavonoid interactions with Cu(II) ions: Implications for their antioxidant properties
J.E. Brown1, H. Khodr2, R.C. Hider2, and C.A. Rice-Evans1
1International Antioxidant Research Center UMDS-Guy¹s Hospital, London, SE1 9RT and 2Department of Pharmacy, King¹s College, University of London, London, SW3 6LX, United Kingdom

Effect of ACEi on antioxidant defenses
Elena M.V. de Cavanagh1, Fernando Carrasquedo1, Daniel Scrivo2, A. Wassermann2, Leon Ferder3, Felipe Inserra3, Cesar G. Fraga1
1Physical Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires; 2CENHA; 3Institute of Biomedical Research-UHABI. Buenos Aires, Argentina

HIV-1 TAT decreases intracellular glutathione content in human Jurkat T cells
Jinah Choi and Henry Jay Forman
Department of Molecular Pharmacology & Toxicology, School of Pharmacy, University of Southern California, Los Angeles, California

Structure and regulation of gpx2 gene expression by selenium in mouse intestine and colon
Fong-Fong Chu*, R. Steven Esworthy*, Weiya Wang¶, Leslie Lee*, and Ye-Shih HoÝ
*Department of Medical Oncology, ¶Department of Neuroscience, City of Hope Beckman Research Center, Duarte, CA 91010, and ÝInstitute of Toxicology, Wayne State University, Detroit, MI 48201


Posters II

Effects of chronic a-lipoic acid treatment on diabetic deficits in corpus cavernosum endothelial responses and non-adrenergic non-cholinergic innervation in rats
Mary A. Cotter, Alan Keegan, and Norman E. Cameron
Biomedical Sciences, Aberdeen University, Scotland, United Kingdom

The production of reactive oxygen intermediates during the induction of apoptosis by cytotoxic insults
Thomas G. Cotter and Adrian J. McGowan
Tumour Biology Laboratory, Department of Biochemistry, University College Cork, Ireland

Topical melatonin in combination with vitamins E and C protects skin from UV-induced erythema: a human study in vivo
F. Dreher1, B. Gabard2, D.A. Schwindt1, H.I. Maibach1
1University of California, School of Medicine, Department of Dermatology, San Francisco, U.S.A., 2Spirig Ltd., Department of Biopharmacy, Pharmaceuticals, Egerkingen, Switzerland

Rat retina light degeneration: Protective effects of EGb 761 and DMTU on the retinal function
Marie-Thérèse Droy-Lefaix*, Isabelle Ranchon**, Jacques Cluzel**, Michel Doly**
*IPSEN Institute, Paris & ** Biophysics Lab., INSERM U. 71, Clermont Ferrand, France

Are antioxidants health effective or preventing disease progeny. The task of validity
Stellan Eriksson
Occupational Health Center, S-613 30 Oxelosund, Health Center Järna, S-153 00 Järna, Sweden

Regular training improves plasma antioxidant status in young soccer players
Pablo A. Evelson1, Fernando D. Brites2, Marina García Christiansen2, María F. Nicol1, Regina W. Wikinski2, and Susana F. Llesuy1
1Cátedra de Química General e Inorgánica y 2Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina

Antioxidant activity of different phenolic fractions separated from an Italian red wine
Andrea Ghiselli*, Mirella Nardini*, Alessandro Baldi**, and Cristina Scaccini*
*Istituto Nazionale della Nutrizione, Via Ardeatina 543 00178 Roma and **Dipartimento di Scienze Farmaceutiche, University of Florence, Via Gino Capponi 9 50121 Firenze - Italy

Bound malondialdehyde: Bioavailability of the N-2-propenals of lysine
Julio Giron-Calle1,2, Valentina Ruiz-Gutierrez1, Manuel Alaiz1, and Eduardo Vioque1
1Instituto de la Grasa y sus Derivados (Consejo Superior de Investigaciones Cientificas), Seville, Spain; 2 University of Southern California, Los Angeles, CA

Regulation of focal adhesion kinase (p125FAK) phosphorylation in ROS-stimulated endothelial cells
Alexia Gozin, Lydie Da Costa, Sophie Faure, Valérie Andrieu, and Catherine Pasquier
INSERM U. 479, CHU Bichat, BP16 75870 Paris cedex 18, France

Poly-ADP-ribose mediated regulation of the 20S proteasome
Tilman Grune, Nicolle Sitte, and Oliver Ullrich
Humboldt University Berlin


Posters III

Menadione induces mitochondrial gene biogenesis in HeLa cells. Is mitochondrial signaling to the nucleus mediated by reactive oxygen species?
Javier Guerrero, Ubaldo Soto, Soledad Miranda, Rocio Foncea, and Federico Leighton
Laboratorio de Citología Bioquímica y Lípidos, Facultad de Ciencias Biológicas, Pontificia Universidad. Católica de Chile, Casilla 114-D, Santiago, Chile

An oxidative stress model of glutamate cytotoxicity in HT-4 hippocampal cells
Derick Han*, Chandan K. Sen, Michael S. Kobayashi, Sashwati Roy, Enrique Cadenas*, and Lester Packer
*Department of Molecular Pharmacology & Toxicologyf, School of Pharmacy, University of Southern California, Los Angeles, and Department of Molecular and Cell Biology, University of California, Berkeley, CA , USA

Antioxidative potential of different antioxidant components and biological tissues measured by the reduction of nitroxide radicals and DPPH
Th. Herrling, J. Fuchs*, and N. Groth#
FOM - University of Applied Science and Technology Berlin, *Johann-Wolfgang Goethe University Frankfurt, #Galenus Inc. Berlin

a-Phenyl-t-butyl-nitrone (PBN) stabilizes free radicals and inhibits NF-kB activation offering protection against the onset of insulin-dependent diabetes mellitus (IDDM)
E.Ho, G. Chen and T.M. Bray
The Ohio State University, Columbus, OH 43210

Free radical scavenging activity of fermented papayapreparation and its effect on lipid peroxide level and superoxide dismutase activity in iron-induced epileptic foci of rats
Katsuki Imao, H. Wang*, M. Komatsu** and M. Hiramatsu**
SAIDO Co., 2-13 3-chome, Befu, Jyonan-ku Fukuoka 814-0104, *Department of 1st Biochemistry, Yamagata University, School of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-2331 and **Institute for Life Support Technology, Yamagata Technopolis Foundation, 2-2-1 Matsuei, Yamagata 990-2473, Japan

S-Nitrosyl glutathione causes both protein S-nitrosylation and protein S-glutathiolation when analyzed by thin gel isoelectricfocusing
Yanbin Ji, Helmut Sies, and James A.Thomas
Deptartment of Biochemistry, Iowa State University, Ames, IA. and Institut für Physiologische Chemie I, Heinrich-Heine Universität, Dusseldorf, Germany

Superoxide anion and hydroxyl radical scavenging activities of vegetable extracts using electron spin resonance
T. Kaneyuki1, Y. Noda2, M. G. Traber2, A. Mori2, and L. Packer2
1Faculty of Health and Welfare Science, Department of Nutritional Science, Okayama Prefectural University, Okayama, Japan, 2Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, U.S.A.

Effects of magnetic fields on the accumulation of thiobarbituric acid reactive substances induced by iron salt in the rat brain homogenate
Hideaki Kabuto, Isao Yokoi, Nihei Yamamoto, Norio Ogawa, and Akitane Mori*
Department of Neuroscience, Institute of Molecular & Cellular Medicine, Okayama University School of Medicine, Okayama, Japan, and *Department of Molecular and Cell Biology, University of California, Berkeley, California, USA

Tyrosine phosphorylation involvement in ADP mediated NF-kB activation
Nalini Kaul, Jinah Choi, and Henry Jay Forman
Department of Molecular Pharmacology & Toxicology, School of Pharmacy, University of Southern California, Los Angeles, CA-90033

Anethole dithiolethione (Sulfarlem) protects against oxidative stress induced cytotoxicity in human Jurkat T cells
Savita Khanna, Chandan K. Sen, Sashwati Roy, Marie-Odile Christen, and Lester Packer
Membrane Bioenergetics Group, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200 and Solvay Pharma, Suresnes Cedex 92151, France


Posters IV

Effects of singlet oxygen and UVA on MAP kinases in human dermal fibroblasts
Lars-Oliver Klotz, 1Freimut Schliess, Karlis Briviba, and Helmut Sies
Heinrich-Heine-Universität Düsseldorf, Institut für Physiologische Chemie I and 1Klinik für Gastroenterologie, Hepatologie und Infektiologie, Moorenstr. 5, D-40225 Düsseldorf, Germany

Serum a-tocopherol and selenium and the risk of coronary heart disease
Paul Knekt1, Antti Reunanen1, Arpo Aromaa1, and Jukka Marniemi2
1National Public Health Institute, Helsinki, 2Social Insurance Institution, Turku, Finland

Ginkgo biloba extract (EGb 761) protects HT-4 neuronal cells against glutamate induced loss of cell viability
Michael S. Kobayashi, Derick Han, Chandan K. Sen, Sashwati Roy, and Lester Packer
Department of Molecular and Cell Biology, University of California at Berkeley

Regulation of Inducible nitric oxide synthase expression in IFN-g-treated macrophages by Bio-Normalizer
Hirotsugu Kobuchi and Lester Packer
Environmental Energy Technologies Division, Lawrence Berkeley National Laboratory, Membrane Bioenergetics Group, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200 USA

Aging versus the concept of reliability: Qualification and qantification of the antioxidant intervention
Vitali Koltover
Institute of Chemical Physics, RAS, Chernogolovka, Moscow Region, Russia

Superoxide and nitric oxide production by leukocytes of diabetic patients. Effects of natural antioxidant bio-normalizer (BN)
L. Korkina*, E. Ostrachovitch§, E. Michal'chik*, and I. Afanas'ev§
*Russian Institute of Pediatric Hematology; §Vitamin Research Institute, Moscow, Russia

Critical interactions of natural antioxidants during low density lipoprotein oxidation
João Laranjinha*,+, Otilia Vieira*, and Enrique Cadenas+
*Center for Neurosciences and Fac. Pharmacy, University of Coimbra, Portugal, +University of Southern California, Los Angeles, CA, USA

Oxidative stress and LDL oxidation in patients with non-insulin-dependent diabetes mellitus (NIDDM)
Janne Leinonen1, Terho Lehtimäki2, Vappu Rantalaiho2, Shinya Toyokuni3, Tiina Solakivi2, and Hannu Alho4
1University of Tampere, Tampere, Finland; 2Tampere University Hospital, Tampere, Finland; 3Kyoto University, Kyoto, Japan; 4National Public Health Institute, Helsinki, Finland

Stable carbon isotope analysis of Lutein using gas isotope ratio mass spectrometry
Y. Lihang, W.S. Trahanovsky, Y. Liang, R.E. Serfass, and W.S. White
Department of Food Science & Human Nutrition and Department of Chemistry, Iowa State University, Ames, IA 50011

Coordinate regulation of g-glutamylcysteine synthetase catalytic and regulatory subunit gene expression in rat lung epithelial L2 cells by 4-hydroxynonenal
Rui-Ming Liu, Lin Gao, Jinal Choi, and Henry Jay Forman
Department of Molecular Pharmacology & Toxicology, School of Pharmacy, University of Southern California, Los Angeles, California


Posters V

Effect of haloperidol on brain mitochondrial respiration and nitric oxide and superoxide production
Silvia Lores Arnaiz, María Florencia Coronel, and Alberto Boveris
Laboratory of Free Radical Biology. School of Pharmacy and Biochemistry, University of Buenos Aires, Jun¹n 956, 1113 Buenos Aires, Argentina

Oleic acid induces PKC dependent generation of reactive species in vascular smooth muscle cells from rat aorta
G. Lu, E.L. Greene, T. Nagai,, and B.M. Egan
Departments of Medicine, Clinical Pharmacology, and Pharmacology Medical University of South Carolina, Charleston, South Carolina

Oxidative status of rat sperm cells exposed to iron
Florencia Lucesoli, Marina Caligiuri, and Cesar G. Fraga
Physical Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina

Ascorbic acid availability and metabolism decline with age in rat hepatocytes. Partial reversal with (R)-a-lipoic acid supplementation
Jens Lykkesfeldt, Tory M. Hagen, Vladimir Vinarsky and Bruce N. Ames
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720

S-Glutathiolation of H-Ras in NIH 3T3 cells
Robert J. Mallis and James A. Thomas
Department of Biochemistry and Biophysics, Iowa State University, Ames, IA

Effect of cellular supplementation with sodium selenite on the reduction of a-lipoic to dihydrolipoic acid in the human Jurkat T cell line: a role of thioredoxin reductase
Lucia Marcocci1,2, Laura McLaughlin1, Michael Kobayashi1, and Lester Packer1
1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. 2Department of Biochemical Sciences ³A. Rossi Fanelli² and CNR Center of Molecular Biology, University of Rome ³La Sapienza² Rome, Italy

Bionormalizer, a novel natural antioxidant, prevents the abstinence-induced lipoperoxidation in moderate drinkers
*F. Marotta, P. Safra, H. Tajiri, and ÝI. Reizakovic
GI service, S. Anna Hospital, Como, Italy; * Econum Lab., Villeneuve d¹Ascq, France; / Natl. Cancer Center East, Chiba, Japan; ÝLiver Unit, Niguarda Hospital, Milano, Italy

Free radical production during the reaction of cytochrome P450 with linoleic acid hydroperoxide
Ronald P. MasonÝ, David P. BarrÝ, Aldo Tomasi*, and Cristina Rota*
*Department of Biomedical Sciences, University of Modena, via Campi 287, 41100 Modena, Italy and ÝNational Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, 27709, USA

The fate of the oxidizing tyrosine phenoxyl radical in the presence of glutathione and ascorbate: Implications for the radical sink hypothesis
Ronald P. Mason*, Herbert J. Sipe§, Jr., David P. BarrÝ, Jose G. Martinez*, and Bradley E. Sturgeon*
Laboratory of Pharmacology and Chemistry, National Institute of Environmental Sciences, National Institutes of Health, Research Triangle Park, NC 27709, §Hampden-Sydney College, Department of Chemistry, Hampden-Sydney, VA 23943, ÝBruker Instruments, 19 Fortune Drive, Manning Park, Billerica, MA 01821

Site-specific spin trapping of tyrosine radicals in the oxidation of metmyoglobin by hydrogen peroxide
Ronald P. Mason, Richard A. Tshcirret-Guth, H. Ewa Witkowska, Yang C. Fann, David P. Barr, Paul Ortiz de Montellano, and Michael R. Gunther
Laboratory of Pharmacology and Chemistry, National Insitute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, Department of Pharmaceutical Chemistry, University of Southern California, San Francisco, CA 94143-0446, and Children¹s Hospital Oakland Research Institute, Oakland, CA


Posters VI

Characterization of the Oxidative Responsive Element (ORE) in the human thioredoxin promoter
H. Masutani, M. Ueno, Y. Yamaguchi, T. Sasada, and J. Yodoi
Institute for Virus Research, Kyoto University 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-01, Japan

Direct evidence for catalase as the predominant H2O2­removing enzyme in human erythrocytes
Sebastian Mueller *, Hans-Dieter Riedel, and Wolfgang Stremmel
*Department of Molecular Pharmacology and Toxicology, University of Southern California, Los Angeles, CA, USA, and Department of Internal Medicine IV, University of Heidelberg, Heidelberg, Germany

Role of p53 in the induction of p21 by redox-cycling anticancer quinones
Sebastian Mueller, Ray-Chang Wu*, Axel H. Schönthal*, and Enrique Cadenas
Department of Molecular Pharmacology and Toxicology, School of Pharmacy, *Department of Molecular Microbiology and Immunology, School of Medicine, University of Southern California, Los Angeles, CA 90033

Tetradecylthioacetic acid a 3-thia fatty acid, is a novel bioactive compound. Besides being an antioxidant, it changes the plasma profile from atherogenic to cardioprotective
Ziad A. Muna, Lise Madsen, and Rolf K. Berge
Department of Clinical Biology, Division of Biochemistry, University of Bergen, Haukeland University Hospital, N-5021 Bergen, Norway

The flavonoid, Quercetin, downregulates HIV-1 entry coreceptor, CCR5, gene expression by normal peripheral blood mononuclear cells (PBMC)
M.P.N. Nair, *C. Kandaswam, A. Sweet, and S.A. Schwartz
Department of Medicine, State University of NY at Buffalo, Buffalo, NY 14203 and Canandaigua West Tech.Ctr, Madera,CA 93639*

Effect of caffeic acid on t-BOOH induced oxidative stress in U937
M. Nardini, §E. Piccolella, P. Pisu, and C. Scaccini
National Institute of Nutrition, Rome; §Department of Cellular and Development Biology, University ³La Sapienza², Rome, Italy

The respiratory burst in activated rat neutrophils: Effects of lipoic acid and magnetic fields
Y. Noda, R.P. Liburdy*, A. Mori, and L. Packer
University of California, Berkeley, CA, USA and the *Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

Nitric oxide scavenging activity of melatonin and its presursors
Y. Noda, A. Mori, and L. Packer
251 LSA, Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, U.S.A.

Evaluation of a1-adrenoceptor antagonist and DL-a-lipoic acid on diabetes-induced changes in retinal NAD(P)-redox status: evidence against "pseudohypoxia?"
I.G. Obrosova, M.J. Stevens, and D.A. Greene
Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA

Chloramines modulate protein kinase C activity
Tetsuya Ogino, Hirotsugu Kobuchi, Chandan K. Sen, Sashwati Roy, Lester Packer, and John J. Maguire
Department of Molecular and Cell Biology and Environmental Energy Technologies Division Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720


Posters VII

The effect of dietary flavonoids and phenolics on the formation of nitrotyrosine by acidic nitrite
Ceri Oldreive, Catherine Rice-Evans, and George Paganga
International Antioxidant Research Centre, UMDS-Guyís Hospital, London, SE1 9RT, United Kingdom

Stereospecific effects of lipoic acids on mammalian pyruvate dehydrogenase complex
M.S. Patel, Y.S. Hong, S.J. Jacobia, and L. Packer
Department of Biochemistry, State University of New York, Buffalo, NY 14214 and Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720

Involvement of NAD(P)H oxidase in 5-HT-1A receptor-activated extracellular signal-regulated protein kinase
D. Pendergrass, S. Pinckney, Y. Mukin, M. Garnovskoya, J.R. Raymond*, and E.L. Greene*
Medical University of South Carolina, Charleston, South Carolina

Failure of heparin-coated circuits to reduce granulocytes activation and antioxidants decrease during cardiopulmonary bypass. A clinical study
J. Pincemail, R. Larbuisson, F. Blaffart, B. Abdallaah, S. Simon, R. Limet, and J.O. Defraigne
Universtiy of Liège - CHU, Dept of Cardiovascular Surgery, CREDEC Sart Tilman 4000 Liège, Belgium

The peroxisome proliferator-activated receptor a regulates cellular redox state in aging
Matthew E. Poynter and Raymond A. Daynes§
Department of Pathology, University of Utah, Salt Lake City, Utah 84132 and §Geriatric Research, Education and Clinical Center, Veterans Affairs Medical Center, Salt Lake City, Utah 84112

Impairment of mitochondrial function by transient hypoxia and/or calcium
L. Schild, I. Wiswedel, F. Plumeyer, T. Reinheckel, and W. Augustin
Institute of Clinical Chemistryf, Department of Pathological Biochemistry, Otto-von-Guericke University, Magdeburg, Germany

Increasing the Intracellular pH of Unfertilized Sea Urchin Eggs Causes An Increase in Reduced Pyridine Nucleotides and Initiates Movement of the Nucleus, DNA Synthesis, and Nuclear Envelope Breakdown
Beth Schomer and David Epel
Hopkins Marine Station, Stanford University, Pacific Grove CA 93950

Role of the alcoholic and non alcoholic fraction in the antioxidant effect of wine
M. Serafini, G. Maiani, and A. Ferro-Luzzi
Human Nutrition Unit, National Institute of Nutrition, Rome, Italy

Oxidative stress in patients with obstructive lymphoedema
Werner G. Siems, Rainer Brenke, Alexander Beier, Eberhard Conradi, and Tilman Grune
Herzog-Julius Hospital of Rheumatology & Orthopaedics, D-38667 Bad Harzburg; Hospital Simbach, D-84359 Simbach; Clinics for Physical Therapy & Rehabilitation, Medical Faculty, Humboldt University, D-10098 Berlin; Germany

Oxidative stress in hemodialysis patients treated with a dialysis membrane which has a-tocopherol bonded to its surface
O.Sommerburg1, K.Sostmann2, T.Grune3, G.Filler2, and J.H.H.Ehrich4
1University Children¹s Hospital of Heidelberg; 2Dept. of Pediatric Nephrology, 3Dept. of Physiotherapy & Rehabilitation, University Hospital Charite, Berlin; Dept. of Pediatrics, Medical School Hannover, Germany


Posters VIII

Oxidation of selective Met residues of apoAI and apoAII by lipid hydroperoxides is an early event during HDL oxidation and can be accelerated by vitamin E
R. Stocker, A.R. Waldeck, P.K. Witting, K.E. RyeÝ, J.K. Christison, M. Raftery¶, and B. Garner
Biochemistry and ¶Immunology Groups, The Heart Research Institute, Sydney, and ÝRoyal Adelaide Hospital, Adelaide, Australia

The effects of ascorbic acid deficiency on the human aorta endothelial cells
Nozomu Tamai and Takao Fujinami
Internal Medicine, Nagoya City University Medical School, Nagoya, Japan

Generation of a-tocopherol transfer protein knockout mice
Yuko Terasawa, Sylvaine Cases, Maret G. Traber, Lester Packer, and Robert V. Farese, Jr.
University of California, Berkeley and San Francisco

The effects of cold acclimation on the activity levels of lactate dehydrogenase and its isoenzymes in various tissues of the rat
S.E. Terblanche, T.C. Masondo, and W. Nel
Department of Biochemistry and Microbiology, University of Zululand, Private Bag X1001, Kwa-Dlangezwa 3886, South Africa

Glutamate­induced apoptosis in HT-4 neuronal cells: Mechanisms and the role of antioxidants
Oren Tirosh, Chandan K. Sen, Sashwati Roy, Michael S. Kobayashi, and Lester Packer
Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200

Spatiotemporal measurement of free radical elimination in the abdomen using an in vivo ESR-CT imaging system
*Hitoshi Togashi, *Haruhide Shinzawa, §Tateaki Ogata, *Taku Matsuo, *Shigeru Ohno, *Koji Saito, *Nobuo Yamada, ¶Hidekatsu Yokoyama, ¶Hiroyuki Noda, ÝKazuo Oikawa, ¶Hitoshi Kamada, and *Tsuneo Takahashi
*The Second Department of Internal Medicine, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-23, §Department of Materials Science and Engineering, Faculty of Engineering, Yamagata University, Yonezawa 992, ¶Institute for Life Support Technology, Yamagata Technopolis Foundation, 2-2-1 Matsuei, Yamagata 990, ÝYamagata Research Institute of Technology, 2-2-1 Matsuei, Yamagata 990, Japan

Lens mitochondrial fragmentation by R-lipoate is concentration- dependent, in protection against glucose-induced diabetic cataractogenesis in vitro
J.R. Trevithick, V. Bantseev, and T. Dzialoszynski
Department of Biochemistry, Faculty of Medicine, The University of Western Ontario, London, Canada

Superoxide radical scavenging activity of ancient dye Shikonin studied by electrochemical ESR analysis
T. Tsujita,2, S. Okamoto2, K. Tajima3, K. N. Azuma1, M. Kohno4 K. Makino5, and K. Ishizu1
1Department of Chemistry, Faculty of Science, Ehime University, Matsuyama 790; 2Coating Division, NOF Corporation, Totsuka-ku, Yokohama 244; 3Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku Kyoto 606; 4Application Center, Analytical Division, JEOL Ltd., Akishima, Tokyo 196; 5Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611, Japan

Reactive oxygen species and nitric oxide production by human blood mononuclear cells
Laura Valdez, Silvia Lores Arnaiz, and Alberto Boveris
Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

The physical state of membranes affects the rate of lipid oxidation. Effect of metals
Sandra V. Verstraeten and Patricia I. Oteiza
Departamento de Química Biológica, IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina


Posters IX

Sebaceous gland excretion is a major pathway for facial delivery of vitamin E to the human skin barrier
S.U. Weber, J.J. Thiele , J. Choi, and L. Packer
251 LSA, Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, USA

The redox state of Polyethylene glycol 20000 Dalton (PEG20L) correlates with heart function following 24 hours of heart preservation
W. N. Wicomb
Department of Organ Preservation, California Pacific Medical Center R310, 2200 Webster St., San Francisco, CA 94115

Protein carbonyls in plasma and bronchial lavage from critically ill sepsis and trauma patients as indicators of early oxidative injury
Christine C. Winterbourn, Hendrikje Buss, Timothy P. Chan, Matthew Clarke*, and Graham Hill*
Department of Pathology, Christchurch School of Medicine, Christchurch, and *Department of Surgery, University of Auckland School of Medicine and Health Sciences, Auckland, New Zealand

Lipid and protein oxidation during oxidative stress in isolated mitochondria
I. Wiswedel, T. Reinheckel, D. Hirsch, H. Noack, and W. Augustin
Institute of Clinical Chemistry, Department of Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Germany

Pathogenic frataxin expansions confer oxidant and iron sensitivity to human fibroblasts which is rescued by chelators of iron and calcium, and inhibitors of apoptosis
Alice Wong1, Franco Taroni2, Cinzia Gellera2, and Gino Cortopassi1
1Department of Molecular Biosciences, University of California, Davis, CA 96516 and 2Department of Biochemical Genetics, Instituto Neurologico ³Carlo Besta², Milan, Italy

Copper/zinc superoxide dismutase and catalase are increased in nitric oxide-resistant HL-60 variant cells
Munehisa Yabuki*Ý, Rumi Ishisaka*, Tatsuji YasudaÝ, Tamotsu Yoshioka*, and Kozo Utsumi*
*Institute of Medical Science, Kurashiki Medical Center, Kurashiki 710, Japan and ÝDepartment of Cell Chemistry, Institute of Molecular and Cell Biology, Okayama University Medical School, Okayama 700, Japan

ESR study of UV-irradiated lipoic acid
A. Yamanaka*,Y. Noda¶, N. Azuma*, A. Mori¶, and L. Packer¶
*Department of Chemistry, Faculty of Science, Ehime University, ¶Membrane Bioenergetics Group, Lawrence Berkeley Laboratory, University of California, USA

The effects of iron and vitamin C co-supplementation on platelet function and oxidative DNA damage in healthy volunteers
Min Yang1, Almas Rehman2, Clifford S. Collis1, George Paganga1, Barry Halliwell2, and Catherine Rice-Evans1
1International Antioxidant Research Centre at UMDS Guyís Hospital, London Bridge, London SE1 9RT, and 2International Antioxidant Research Centre at Kings College, Chelsea Campus, London SW3 6LX, United Kingdom

Alternate magnetic fields potentiate monoamine oxidase activity in the brain
Isao Yokoi, Hideaki Kabuto, Nihei Yamamoto, Norio Ogawa, and Akitane Mori*
Department of Neuroscience, Institute of Molecular & Cellular Medicine, Okayama University School of Medicine, Okayama, Japan, and *Department of Molecular and Cell Biology, University of California, Berkeley, California, USA

Evidence of redox-mediated pathophysiology in rheumatoid arthritis: synergistic activation of NF-kB by TNF and thioredoxin in the cultured rheumatoid synovial fibroblasts
Shinichi Yoshida1,2, Katsuko Uno3, Nobuo Matsui2, and Takashi Okamoto1
Departments of 1Molecular Genetics and 2Orthopedics, Nagoya City University, Nagoya and 3Louis Pasteur Center of Medical Research, Kyoto, Japan


Posters X

A new pathway to generate nitric oxide by xanthine oxidase
Z. Zhang, D. Naughton, P. Winyard, D. Blake, and M. Symons
Bone and Joint Research Unit, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London E1 2AD, UK

Antioxidant or prooxidant: paradoxical effect of 1,10-phenanthroline on hydrogen peroxide-induced cytotoxicity in Escherichia coli
Ben-Zhan Zhu and Mordechai Chevion
Department of Cellular Biochemistry, Hebrew University-Hadassah Schools of Medicine and Dental Medicine, Jerusalem 91120, Israel

Organic Fenton reaction: the production of hydroxyl radical by penta-chlorophenol metabolites in the presence of H2O2
Ben-Zhan Zhu and Mordechai Chevion
Department of Cellular Biochemistry, Hebrew University-Hadassah Schools of Medicine and Dental Medicine, Jerusalem 91120, Israel

The role of transition metal ions and chelators on the toxicity of pentachlorophenol and its main metabolite, tetrachlorohydroquinone
Ben-Zhan Zhu, Svetlana Schechtman, and Mordechai Chevion
Department of Cellular Biochemistry, Hebrew University-Hadassah Schools of Medicine and Dental Medicine, Jerusalem 91120, Israel

Hemoglobin catalyses LDL-Apo B modification during extracorporeal circulation of blood and plasma
Ouliana Ziouzenkova1, Liana Asatryan1, Mary Lou Wratten2, Ciro Tetta2, and Alex Sevanian1
1 Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, USA, and 2Clinical and Laboratory Research Department, Bellco S.p.A., Italy

The novel radical scavenger Imidate inhibits neutrophil activation and decreases infarct size with enhanced recovery of contractile function in the postischemic heart
Jay L. Zweier, Penghai Wang, Emad A. Mikhail, and Howard Elford*
EPR Center and Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, and *Molecules For Health, Richmond, VA

Evidence for chronic oxidative stress in Down syndrome
Slobodan V. Jovanovic and Kent MacLeod
International Center for Metabolic Testing, 1305 Richmond Road, Ottawa, ON, Canada K2B 7Y4


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